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Test Code PLHBB Plasma Free Hemoglobin, Plasma


Specimen Required


Collection Container/Tube: Lavender top (EDTA)

Submission Container/Tube: Plastic vial

Specimen Volume: 2 mL

Collection Instructions:

1. Centrifuge and transfer plasma to a plastic vial within 2 hours of collection.

2. Results could be falsely elevated due to artifactual red blood cell lysis if not centrifuged within 2 hours of collection.


Forms

If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen.

Useful For

Determining whether hemolysis is occurring such as from:

-Transfusion reaction

-Mechanical fragmentation of red blood cells

-Relative comparison to baseline levels in extracorporeal membrane oxygenation and centrifugal ventricular assist device patients to assess pump disruption

Method Name

Spectrophotometry (SP)

Reporting Name

Plasma Free Hemoglobin, P

Specimen Type

Plasma EDTA

Specimen Minimum Volume

1.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma EDTA Refrigerated (preferred) 20 days
  Frozen  30 days
  Ambient  4 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK

Reference Values

TOTAL PLASMA HEMOGLOBIN

≥12 months: 0.0-15.2 mg/dL

Reference values have not been established for patients who are younger than 12 months of age.

 

OXYHEMOGLOBIN

≥12 months: 0.0-12.4 mg/dL

Reference values have not been established for patients who are younger than 12 months of age.

Day(s) Performed

Monday through Sunday

Report Available

1 to 3 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

83051

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PLHBB Plasma Free Hemoglobin, P 87433-9

 

Result ID Test Result Name Result LOINC Value
31970 Total Plasma Hemoglobin 721-1
31971 Oxyhemoglobin 87437-0