Test Code CAFF Caffeine, Serum
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL Serum
Collection Instructions:
1. Within 2 hours of collection, centrifuge the specimen.
2. For serum gel tubes, serum may sit on the gel or be aliquoted into a plastic vial.
3. For red top tubes, aliquot serum into a plastic vial immediately after centrifugation.
Useful For
Monitoring caffeine therapy in neonates
Assessing caffeine toxicity in neonates
Method Name
Enzyme Multiplied Immunoassay Technique (EMIT)
Reporting Name
Caffeine, SSpecimen Type
SerumSpecimen Minimum Volume
Serum: 0.25 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Frozen (preferred) | 28 days |
| Ambient | 72 hours | |
| Refrigerated | 72 hours |
Reject Due To
| Gross hemolysis | Reject |
Reference Values
Therapeutic: 8.0-20.0 mcg/mL
Critical value: ≥30.0 mcg/mL
Day(s) Performed
Monday through Saturday
Report Available
Same day/1 to 2 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
80155
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| CAFF | Caffeine, S | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 8754 | Caffeine, S | 3422-3 |
Forms
If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.